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Identification and control of die-back disease of mulberry trees using a combination of formulations based on ethane peroxic acid in green space of Tehran
Volume 2, Issue 1, 2020, Pages 3 - 9
Author(s) : Zeynab Keykhosravi* 1 , Nima Khaledi 2 , Asghar Saleh 3 , Farzad Rahmani 4 , Somayeh Seyfouri 5
1 Green area education and counseling research center of the municipality of district 17 Tehran,Iran
2 Seed and Seedling Registration and Certification Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
3 Green Space Education and Consulting Research Center, District 17, Tehran, Iran
4 Technical manager of biological and non-chemical control of Pak Gostar Parand Company, Tehran, Iran
5 Green Space Education and Consulting Research Center, District 17, Tehran, Iran
Abstract :
Branch die-back disease caused by Nattrassia mangiferae is one of the most important fungal diseases of berries. The disease is most commonly seen in urban green space in high humidity and high temperatures during most seasons when trees are exposed to the sun. In this study, the causative fungus was sampled and isolated from mulberry trees on Matin Street in Tehran Municipality, District 17. Proof of pathogenicity of the causative fungus was performed on cut branches of mulberry and biennial seedlings. They were identified based on morphological and molecular characteristics (based on nucleotide sequencing of ITS-rDNA regions amplified with ITS1 and ITS4 primers). The composition formulated on the basis of ethane peroxic acid 20% prepared by Pak Gostar Parand Company against the fungus causing the disease in laboratory conditions and urban green space was studied in a completely randomized design with 4 replications. The results of laboratory studies on the mixing method in PDA culture medium showed that at a concentration of 3000 ppm it was 91.25%. While at concentrations above 4000 ppm, it completely prevented the growth of mycelial fungi. Moreover, the results of comparing the effect of the composition formulated with Berdofix fungicide on the branches of mulberry trees in urban green space showed that there was a statistically significant difference in reducing the symptoms of die-back disease and the composition formulated at a concentration of 5000 ppm compared to Berdofix which inhibited the spread of the disease in the berry trees of the municipality of District 17. Therefore, this combination can be used to control die-back disease in line with environmental goals.
Branch die-back disease caused by Nattrassia mangiferae is one of the most important fungal diseases of berries. The disease is most commonly seen in urban green space in high humidity and high temperatures during most seasons when trees are exposed to the sun. In this study, the causative fungus was sampled and isolated from mulberry trees on Matin Street in Tehran Municipality, District 17. Proof of pathogenicity of the causative fungus was performed on cut branches of mulberry and biennial seedlings. They were identified based on morphological and molecular characteristics (based on nucleotide sequencing of ITS-rDNA regions amplified with ITS1 and ITS4 primers). The composition formulated on the basis of ethane peroxic acid 20% prepared by Pak Gostar Parand Company against the fungus causing the disease in laboratory conditions and urban green space was studied in a completely randomized design with 4 replications. The results of laboratory studies on the mixing method in PDA culture medium showed that at a concentration of 3000 ppm it was 91.25%. While at concentrations above 4000 ppm, it completely prevented the growth of mycelial fungi. Moreover, the results of comparing the effect of the composition formulated with Berdofix fungicide on the branches of mulberry trees in urban green space showed that there was a statistically significant difference in reducing the symptoms of die-back disease and the composition formulated at a concentration of 5000 ppm compared to Berdofix which inhibited the spread of the disease in the berry trees of the municipality of District 17. Therefore, this combination can be used to control die-back disease in line with environmental goals.
Keywords :
mulberry, dieback, formulation, green areas
mulberry, dieback, formulation, green areas