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Induction of callus in hybrid Rose (Rosa hybrida L.) in vitro culture medium
Volume 1, Issue 2, 2019, Pages 132 - 139
1 Master student of Agricultural Engineering (Plant Breeding), Payame Noor University of Mashhad
2 Assistant Professor, Department of Agricultural Engineering, Payame Noor University
3 Assistant Professor, Department of Agricultural Engineering, Payame Noor University
Abstract :
Roses are one of the most beautiful flowers in the world and have a wide variety of uses. The number of rose cultivars in the world is increasing. With the scientific name Rosa hybrida L., hybrid Rose belongs to the Rosaceae family. This plant is an important ornamental and commercial plant in the flower industry, cut flowers, and medicine and perfumery industries. Due to the limitations of propagation by cuttings and other propagation methods of this species, determining the optimal conditions for hybrid rose propagation through tissue culture seems necessary to reduce its propagation costs. For this purpose, a small stem sample containing lateral buds was used for callus formation of hybrid roses. Micro-disinfected samples in MS culture medium containing growth regulators including 6-benzyl amino purine (BAP) with concentrations of 0, 1, 2 and 3 mg/L and naphthalene acetic acid (NAA) at concentrations of 0, 0.1, 0.5 and 1 mg/L were cultured. The experiment was performed as factorial in a completely randomized design with three replications, each of which consisted of 5 micro-samples. The results showed that the highest degree of callus formation was observed in treatments containing micro-samples of hybrid roses at a concentration of 0.5 mg/L NAA and 2 mg/L BAP.
Roses are one of the most beautiful flowers in the world and have a wide variety of uses. The number of rose cultivars in the world is increasing. With the scientific name Rosa hybrida L., hybrid Rose belongs to the Rosaceae family. This plant is an important ornamental and commercial plant in the flower industry, cut flowers, and medicine and perfumery industries. Due to the limitations of propagation by cuttings and other propagation methods of this species, determining the optimal conditions for hybrid rose propagation through tissue culture seems necessary to reduce its propagation costs. For this purpose, a small stem sample containing lateral buds was used for callus formation of hybrid roses. Micro-disinfected samples in MS culture medium containing growth regulators including 6-benzyl amino purine (BAP) with concentrations of 0, 1, 2 and 3 mg/L and naphthalene acetic acid (NAA) at concentrations of 0, 0.1, 0.5 and 1 mg/L were cultured. The experiment was performed as factorial in a completely randomized design with three replications, each of which consisted of 5 micro-samples. The results showed that the highest degree of callus formation was observed in treatments containing micro-samples of hybrid roses at a concentration of 0.5 mg/L NAA and 2 mg/L BAP.
Keywords :
Hybrid Rose, Callus Formation, In Vitro Culture
Hybrid Rose, Callus Formation, In Vitro Culture