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Biological control of Aspergillus niger and Aspergillus flavus isolated from strawberry (Fragaria x ananassa Duch ) by streptomyces sp.
Volume 5, Issue 1, 2022-2023, Pages 28 - 33
1 Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
2 Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
3 Department of Microbiology, Faculty of biological science, North Tehran Branch, Islamic Azad University, Tehran,Iran
Abstract :
Actinobacteria are major producers of antifungal metabolites especially for use in agriculture biocontrol. In this study, the identification of Streptomyces sp. isolated from saline soil was carried out using morphological and biochemical studies. Optimization the culture conditions to produce metabolite was done. Two fungal samples were isolated from decaying strawberry. Their molecular identity was determined. Effects of Antifungal activity of Streptomyces sp. activities was determined by agar dilution method and inhibitory percentage of growth evaluated. Results showed the strain of actinomyces isolate, strongly belongs to the genus Streptomyces. The highest metabolite wieght (1.4 gram) at 32 0C, at pH 6.0, an incubation time of 120h under aerobic conditions with culture medium compounds (yeast extract 0.4%, Dextrose 0.4 %, Pepton 0.5%, NaCl 0.5%) happened. Bioactive metabolite Streptomyces sp. effected on two isolates of Aspergilllus (A. niger and A. flavus) isolate. Concentration of 6 mg / ml of metbolites has 100% growth inhibition, In contrast, mancozeb fungicide had 100% growth inhibition at the same concentration too. Therefore, based on the results achived, metabolite produced by Streptomyces sp. can be used as a biological agent to control the fungi infecting strawberr y and it is a suitable alternative to chemical fungicides.
Actinobacteria are major producers of antifungal metabolites especially for use in agriculture biocontrol. In this study, the identification of Streptomyces sp. isolated from saline soil was carried out using morphological and biochemical studies. Optimization the culture conditions to produce metabolite was done. Two fungal samples were isolated from decaying strawberry. Their molecular identity was determined. Effects of Antifungal activity of Streptomyces sp. activities was determined by agar dilution method and inhibitory percentage of growth evaluated. Results showed the strain of actinomyces isolate, strongly belongs to the genus Streptomyces. The highest metabolite wieght (1.4 gram) at 32 0C, at pH 6.0, an incubation time of 120h under aerobic conditions with culture medium compounds (yeast extract 0.4%, Dextrose 0.4 %, Pepton 0.5%, NaCl 0.5%) happened. Bioactive metabolite Streptomyces sp. effected on two isolates of Aspergilllus (A. niger and A. flavus) isolate. Concentration of 6 mg / ml of metbolites has 100% growth inhibition, In contrast, mancozeb fungicide had 100% growth inhibition at the same concentration too. Therefore, based on the results achived, metabolite produced by Streptomyces sp. can be used as a biological agent to control the fungi infecting strawberr y and it is a suitable alternative to chemical fungicides.
Keywords :
Aspergillus, Biological fungicides, Streptomyces, strawberry
Aspergillus, Biological fungicides, Streptomyces, strawberry